Plant Micro-propagation - An Innovative way to save Endangered Plants
The Micro-propagation process includes 5 different stages, they are 0. Selection of mother plant and its maintenance 1. Initiation and establishment of culture 2. Multiplication of shoots or rapid somatic embryo formation 3. In vitro germination of somatic embryos and/or rooting of shoot 4. Transfer of plant to soil that is sterilized to strength with the presence of CO2.
STAGE 0: Initial process in which the stock plants are selected and grown about 3 months under controlled conditions.
STAGE 1: Initiating and establishing of culture in a suitable medium is achieved. Selection of appropriate explant is important.
STAGE 2: Involves multiplication of shoots or rapid embryo formation from the explant.
STAGE 3: Transfering shoot for rapid development into shoots.
STAGE 4: Transfer of plantlets from the laboratory condition to greenhouse or to land etc.,
METHODS INVOLVED IN MICRO-PROPAGATION
· Meristem culture
· Callus culture
· Suspension culture
· Embryo culture
· Protoplast culture
ADVANTAGES OF MICRO-PROPAGATION
Ø The space required is relatively small.
Ø The initial producer or donor of plant species is comparatively less where the tissue will result in regenerating millions of clones in a year.
Ø The in-vitro stocks can be quickly proliferated at any time of the year. Also, it provides year-round nursery for ornamental, fruit and tree species.
Ø Disease-free plants can be produced.
Ø Production of seeds are numerous in amount.
Ø Stock of germplasm can be stored for years.
Ø Economical maintenance and pathogen-free plants are produced.
Ø Identical plants are produced.
Micro Propagation Calamus huegelianus
In-vitro micropropagation of Calamus huegelianus, an endangered taxon, belongs to the family Arecaceae. Plantation at Western Ghats resulted for the demand. MS and L2 medium supplemented with various growth regulators. L2+2, 4-D and L2+IAA+BAP were the combinations used. Shoots regenerated and rooted in the presence of IAA or BAP.
Micro-Propagation of Eucalyptus
Micro-propagation through axillary proliferation or adventitious shoot proliferation on nodal explants, or both, has been successful. Murashige and Skoog media with low of auxin or cytokinin ratio is most commonly used for shoot multiplication.
Micro-Propagation of Plumbago zeylanica Linn
Medicinal plant Plumbago zeylanica Linn, vegetative shoot buds were established by effective method like plant micro-propagation. Multiple shoots were also proliferated from nodal explants cultured on Murashige and Skoog basal medium supplemented with kinetin or N6-benzyl adenine (0.25-1.0mg/l). Excised shootlets cultured on hormone-free basal medium rooted within 4 to 7 days.